About Us

About Us

Who we are and what we do?

Our research program combines genomics and plant-microbe interactions. Our vision is to combine new technologies, fundamental biology and translational research to make an impact on agriculture and food security.

We are an inter-disciplinary group of people with expertise in comparative genomics and bioinformatics, plant biology, and microbiology. We work on the high performance computer, in the molecular biology lab as well as in the field. Our common goal is to understand the molecular and evolutionary basis for plant-microbe interactions with a focus on plant immunity and plant pathogens.  

Below are examples of ongoing projects in the Krasileva lab:

Evolution and function of plant immune receptors

Plant NLR immune receptors enable plants to recognize diverse pathogens. Plants deploy multiple mechanisms of NLR diversification and regulation. Here is our review on this topic.

We are interested to understand evolution of NLRs on multiple levels: 1) within a single organism. How much diversity is generated in each generation? 2) on the population level. How much immune receptor diversity does a field of grass have? 3) Across species 4) Across kingdoms. We are keen to examine effects of domestication and hybridization on NLR evolution on all these levels.

Our recent evolutionary analyses of NLRs in nine grass species (Setaria, Brachypodium, barley, rice, maize, wheat, and two wild wheat progenitors) demonstrated that a specific class of NLRs is prone to diverse fusions with other genes, often originating from different chromosomes. Such fusions are utilized by NLRs as baits the pathogen-derived molecules. Based on current data, we think that the most likely mechanism for this is non-homologous recombination where a nicking of DNA right after an NLR is repaired with DNA from another genomic location. We are working to uncover the exact mechanism of how NLRs can tolerate new gene fusions, so we can hack this evolutionary mechanism for designing new immune receptor.

In comparison to the Poaceae, other plant families have lower numbers of NLRs. Analysis of nearly 80 plant genomes revealed several independent events of lineage specific NLR contractions. NLR phylogenies across diverse families also revealed conservation of subclasses of NLRs. We propose that these conserved NLRs are candidates for a minimal functional plant immunity.

We have identified maize and Sorghum lineages as a prime candidates for additional evolutionary analyses. Unlike other grasses, maize exhibits a putative reduction in NLR genes. We are currently investigating if this reduction is due to domestication, inbreeding, or extensive balancing selection that maintains fewer loci in each genome.

Mutational genomics to enhance disease resistance

We have previously identified 34 mutant wheat lines with enhanced adult plant resistance against a devastating wheat pathogen called wheat yellow (stripe) rust. Our goal is to identify mutations linked to resistance and clone genes with causative mutations.

We identified our candidate mutant lines directly in the US field trials. We tested and confirmed resistance in a subset of lines across three continents, America (California), Europe (UK) and Africa (Kenya). In the UK, we have been testing resistance for four years confirming broad spectrum resistance against yellow rust in 16 lines. All 34 lines were evaluated in the fields in Kenya (for resistance against stem rust) through collaboration with Durable Resistance in Wheat Gates project.

We deployed exome capture and bulk segregant analysis of mutations in F2 population to map genetic interval co-segregating with resistance in the first mutant line. Wheat has a complex genome, 5x larger than the human genome, and processing wheat genomic data remains a computational challenge. Utilizing the DRAGEN Bio-IT processor (Edico Genomics) available at Earlham Institute, we were able to reduce the processing time from days to minutes per sample.

We have advanced genetic material to the F2/3 generations and are scoring them in the field, aiming to de-cipher the resistance genes within the next year.

The next step after we know what mutations have beneficial effect on disease resistance is introducing them to elite varieties grown across the world. We are taking two complimentary approaches 1) mining natural variation in wheat for alleles similar to our induced changes from projects such as WHEALBI where the data is available on >500 wheat lines representing worldwide and historical diversity 2) introducing precise changes into elite varieties using genome editing.

And of course, we are planning more mutational genomics screens, both in the field and in the lab.

Our overarching research aims

  1. Uncover the natural history of plant-microbe interactions through comparative genomics.
  2. Understand the effects of domestication, adaptation to new environments, polyploidy and inbreeding on plant immunity.
  3. Engineer plant immunity using CRISPR and synthetic biology.